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Induction of B‐cell chronic lymphocytic leukaemia and hairy cell leukaemia like phenotypes by phorbol ester treatment of normal peripheral blood B‐cells
Author(s) -
Visser Lydia,
Poppema Sibrand
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb04349.x
Subject(s) - hairy cell , cd5 , immunophenotyping , cd11c , b cell , chronic lymphocytic leukemia , microbiology and biotechnology , phorbol , phenotype , hairy cell leukemia , biology , stimulation , flow cytometry , immunology , chemistry , leukemia , endocrinology , biochemistry , antibody , protein kinase c , phosphorylation , gene
Summary. To investigate the relationship between normal B‐cells, B‐cell chronic lymphocytic leukaemia (B‐CLL) cells and hairy cell leukaemia (HCL) cells the three cell types were incubated with phorbol myristic acetate (PMA). The parameters studied were morphology, immunophenotype and tartrate resistant acid phosphatase (TRAP). PMA stimulation of B‐cells induced morphological changes as well as CD5, CD11c, B‐ly 7 and TRAP positivity. The cells formed small aggregates, the cell membranes were ruffled and frequently hairy and a small number of cells became plastic adherent and developed dendritic structures. CD5 and CD11c appeared on day 2, imitating a B‐CLL phenotype. On day 3 and 4 a decrease of CD5, an increase of CD11c and the appearance of B‐ly 7 could be seen mimicking an HCL phenotype. The changes in B‐CLL and HCL upon PMA stimulation were mainly morphological; the B‐CLL cells became ruffled and aggregated strongly, while the HCL cells developed dendritic features and became adherent. The immunophenotype of the PMA stimulated HCL cells did not change. The B‐CLL cells remained CD5 positive and did not become B‐ly 7 positive. The findings indicate that although PMA stimulation of normal peripheral blood cells results in CLL‐ as well as HCL‐like phenotypes, similar stimulation of B‐CLL cells does not result in an HCL‐like phenotype.