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Immunohisto‐cytochemical correlation of DAP IV‐CD 26 reactivity with immunblogic markers of lymphocyte activation in human lymphoid tissues
Author(s) -
Cozzi Marzia,
Gloghini Annunziata,
Volpe Rachele,
Carbone Antonino
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb04344.x
Subject(s) - lymphocyte , pathology , microbiology and biotechnology , immunostaining , lymph node , population , immunohistochemistry , lymph , antibody , staining , biology , monoclonal antibody , t lymphocyte , immunology , antigen , medicine , environmental health
Summary. The topographic distribution of the dipeptidylami‐nopeptidase IV (DAP IV‐CD 26) and II (DAP II) positive T‐cell population in six reactive lymph nodes and seven follicular B‐cell non‐Hodgkin's lymphomas (NHL) was analysed with regard to the distribution of activated T‐cells, as visualized by a panel of monoclonal antibodies including Tac‐CD 25, HLA‐DR, OKT 9‐CD 71, ICAM‐1‐CD 54, LFA‐1‐CD 11a. For comparative studies serial frozen sections of the lymph nodes were tested by enzyme histochemistry and immunohistochemistry. In addition, cell suspensions obtained from 10 B‐NHL and interleukin‐2 (IL‐2) activated T‐cells were investigated by a combined cytochemical and immunological method for simultaneous visualization of DAP IV‐CD 26 cytoplasmic activity and surface immunostaining for markers of lymphocyte activation. Both in reactive and lymphomatous lymph nodes the topographic distribution of DAP IV‐CD 26 + and DAP II + lymphocytes was rather similar to that of Tac‐CD 25 + lymphocytes. On the contrary, the DAP IV‐CD 26 and DAP II distribution pattern substantially differed from that of the other immunologic markers. In a cell suspension of IL‐2 activated T‐cells, more than 80% of the cells with a blastic morphology were DAP IV‐CD 26 + : DAP IV‐CD 26 + cells coexpressing Tac‐CD 25, OKT 9‐CD 71, HLA‐DR positivity, relative to the total number of DAP IV‐CD 26 positive cells, were 90.5%, 70.5% and 87% respectively. Only small (not activated) lymphocytes expressed a focal cytoplasmic DAP II positivity. In cell suspensions from 10 cases of B‐NHL the mean percentage of DAP IV‐CD 26 + Tac‐CD 25 + cells was 75.8. Only a small number of DAP IV‐CD 26 + cells coexpressed HLA‐DR, the mean percentage being 9.6. The results support the view that DAP IV‐CD 26 may be considered as a marker of lymphocyte activation; this marker seems to be restricted to T lymphocytes that reside in the T dependent areas of reactive lymph nodes and to non malignant T‐cells surrounding neoplastic follicles of follicular NHL.