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Tissue sources of murine megakaryocyte potentiator: biochemical and immunological studies
Author(s) -
Banu Naheed,
Fawcett Jenny,
Williams Neil,
Giorgio Toni,
Withy Raymond
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb04342.x
Subject(s) - potentiator , megakaryocyte , bone marrow , thrombopoietin , erythropoietin , chemistry , lipopolysaccharide , granulocyte macrophage colony stimulating factor , lung , immunology , interleukin , progenitor cell , biology , medicine , haematopoiesis , cytokine , endocrinology , microbiology and biotechnology , stem cell
Summary. The immunological and biochemical characteristics of murine megakaryocyte potentiator from lung and bone marrow were examined and compared with thrombopoietic stimulatory factor. Biological activity was not neutralized by anti‐erythropoietin, but megakaryocyte potentiator activity from all three sources was abolished or reduced when the preparations were treated with anti‐thrombopoietic stimulatory factor or anti‐interleukin‐6. Megakaryocyte potentiator levels in lung conditioned medium were not found to be enhanced from mice treated with lipopolysaccharide, in contrast to granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) levels. The biochemical properties of murine megakaryocyte potentiator from lung and bone marrow were compared and found to be similar in the elution profiles from anion exchange, gel filtration and reversed phase liquid chromatography. It is concluded that the activities in lung and bone marrow are very similar if not identical, to interleukin‐6.