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Elevated white blood cell synthesis of leukotriene C 4 in chronic myelogenous leukaemia but not in polycythaemia vera
Author(s) -
Stenke Leif,
Samuelsson Jan,
Palmblad Jan,
Dabrowski Lech,
Reizenstein Peter,
Lindgren JanÅke
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb02580.x
Subject(s) - polycythaemia , white blood cell , leukotriene , leukotriene b4 , medicine , immunology , blood cell , gastroenterology , inflammation , asthma
Summary Leukotriene (LT) formation was studied in ionophore A23187‐stimulated white blood cell (WBC) preparations from patients with chronic myelogenous leukaemia (CML; n =14), polycythaemia vera (PV; n =10) and two control groups consisting of patients with non‐malignant inflammatory disease ( n = 4) and normal healthy donors ( n = 25). The synthesized products were identified and quantitated using high‐performance liquid chromatography combined with computerized UV‐spectroscopy. White blood cell preparations from the CML patients produced more LTC 4 (40.2±7.9 pmol/10 6 WBC, mean±SEM) than WBC from the healthy donors (9.0±1.8), P <0.0005. In contrast, the formation of LTB 4 was normal and there was no increase in the total leukotriene synthesis (the sum of LTC 4 , LTB 4 , 20‐OH‐LTB 4 and the Δ 6 ‐ trans ‐isomers of LTB 4 ). The ratio between leukotrienes C 4 and B 4 was strongly elevated in the CML group; 1.67 ± 0.25 v. 0.37±0.07 in the controls, P <0.0005. No significant correlation was observed between the levels of LTC 4 and the number of known LTC 4 producing cells (such as monocytes, eosinophils and basophils) in the CML WBC preparations. In contrast, a correlation was found between the sum of neutrophilic granulocytes and metamyelocytes in these suspensions and the amount of LTB 4 formed; r =0.600. P <0.05 A number of other laboratory or clinical variables of the CML patients (including total white blood cell and platelet counts, differential counts, previous cytotoxic treatment, time from diagnosis, time from last treatment, post study survival and age) did not significantly correlate with the formation of leukotrienes. No abnormality in the production of LTB 4 or LTC 4 was observed in granulocyte and WBC preparations from the patients with polycythaemia vera and non‐malignant inflammatory disease, respectively. The results indicate a selectively increased LTC 4 producing capacity in CML.