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Myelodysplastic syndrome (MDS)‐associated inhibitory activity on haemopoietic progenitor cells
Author(s) -
Ohmori Masami,
Ohmori Seiichi,
Ueda Yasunori,
Tohyama Kaoru,
Yoshida Yataro,
Uchino Haruto
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb02563.x
Subject(s) - granulopoiesis , progenitor cell , peripheral blood mononuclear cell , macrophage , granulocyte , inhibitory postsynaptic potential , cfu gm , bone marrow , colony stimulating factor , immunology , biology , medicine , in vitro , endocrinology , haematopoiesis , stem cell , biochemistry , microbiology and biotechnology
Summary We studied MDS‐associated inhibitory activity, which inhibited colony formation in vitro of granulocyte‐macrophage progenitors (CFU‐GM). Macrophages obtained from MDS bone marrow mononuclear cells (BM‐MNC) when pretreated with granulocyte‐macrophage colony stimulating factor (GM‐CSF) suppressed the growth of normal CFU‐GM. These macrophages were designated as ‘MDS‐derived inhibitory macrophages’. Media conditioned by MDS‐derived inhibitory macrophages (MDS‐CM) also suppressed the growth of normal CFU‐GM. In the MDS‐CM, high levels of prostaglandin E 2 (PGE 2 ) and ferritin were found. However, MDS‐CM did not contain detectable levels of tumour necrosis factor (TNF) or gamma‐interferon (γ‐IFN). Antiserum against human placental ferritin and/or against PGE 2 blocked the haemopoietic inhibitory activity to some extent. These results suggest that inhibitory macrophages may be responsible for the suppression of granulopoiesis in patients with MDS and that the suppression may be mediated by soluble factors including PGE 2 and ferritin.