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Reactivation of HbF synthesis in normal adult erythroid bursts by IL‐3
Author(s) -
Gabbianelli M.,
Pelosi E.,
Labbaye C.,
Valtieri M.,
Testa U.,
Peschle C.
Publication year - 1990
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1990.tb02547.x
Subject(s) - erythroblast , erythropoiesis , erythropoietin , peripheral blood mononuclear cell , immunology , biology , in vitro , granulocyte , fetal hemoglobin , in vivo , microbiology and biotechnology , cytokine , fetus , endocrinology , medicine , anemia , biochemistry , pregnancy , genetics
Summary Reactivation of HbF synthesis has been reported in normal adult erythroblast colonies (‘bursts’) generated by erythroid progenitors (BFU‐E) after seeding peripheral blood mononuclear cells (PBMC) in fetal calf serum‐supplemented (FCS + ) semisolid cultures stimulated by erythropoietin (Ep). Reactivation is almost totally suppressed when: (i) PBMC are grown in optimized FCS ‐ culture or (ii) PBMC are first stringently depleted of monocytes and then plated in FCS + medium (i.e. BFU‐E growth in FCS + Mo ‐ culture). In either case, addition of biosynthetic granulocyte‐macrophage colony stimulating factor (GM‐CSF) induces a dose‐related increase of relative HbF synthesis up to the level in FCS + culture. We report that, in FCS ‐ culture of partially purified adult blood BFU‐E, treatment with biosynthetic interleukin 3 (IL‐3) causes a dose‐related rise of relative HbF production in the bursts. A similar phenomenon is observed in FCS + culture of highly purified BFU‐E. The rise of HbF synthesis is seemingly mediated, at least in part, by a direct effect of IL‐3 at BFU‐E level. It is tentatively concluded that reactivation of HbF in vitro. as well as in a variety of in vivo conditions (i.e. stress erythropoiesis, marrow regeneration), may be at least in part mediated by IL‐3 and GM‐CSF.