Premium
Relationship between the nicotinamide adenine dinucleotide redox potential and the 2,3‐diphosphoglycerate content in the erythrocyte in sickle cell disease
Author(s) -
Lachant Neil A.,
Zerez Charles R.,
Tanaka Kouichi R.
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb07693.x
Subject(s) - nad+ kinase , nicotinamide adenine dinucleotide , chemistry , diphosphoglycerate , glycerol 3 phosphate dehydrogenase , nicotinamide , red cell , biochemistry , medicine , endocrinology , biology , enzyme , hemoglobin
Summary. The percentage of nicotinamide adenine dinucleotide (NAD) in the oxidized form [NAD + /(NAD + and NADH); i.e. the NAD + /NAD T ratio] is increased in the red cell (RBC) in sickle cell disease. We tested the hypothesis that the increased NAD + /NAD T ratio was a determinant of the increased 2,3‐diphosphoglycerate (DPG) content of the SCD RBC. Using normal subjects and individuals with sickle cell disease or autoimmune haemolytic anaemia (AIHA), we observed an inverse relationship between the packed cell volume (PCV) and the RBC DPG concentration ( r =−0·69) and a direct relationship between the RBC NAD + /NAD T ratio and the DPG concentration ( r = 0·74). When the effect of the PCV on DPG was removed using analysis of covariance [DPG ady(PCV) ], the NAD + /NAD T ratio had a significant relationship with the DPG adj(PCV) ( r = 0·50, P < 0·001). In in vitro incubation studies, increasing the NAD + /NAD T ratio significantly increased the DPG content of both normal and AIHA RBC. Conversely, decreasing the NAD + /NAD T ratio decreased the DPG content of normal, AIHA and SCD RBC. Thus, the increased DPG content in the SCD RBC appears to be due, in part, to the increased NAD + /NAD T ratio and is not purely a physiologic response to decreased oxygen carrying capacity.