Premium
N‐RAS mutations in T‐cell acute lymphocytic leukaemia: analysis by direct sequencing detects a novel mutation
Author(s) -
Bareli M.,
Ahuja H.,
And A. Foti,
Cline M. J.
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb07648.x
Subject(s) - exon , mutation , microbiology and biotechnology , biology , genetics , gene , threonine , serine , phosphorylation
Summary. A novel mutation of the N‐RAS gene of T‐ALL blast cells was detected by a direct sequencing of in vitro amplified exon‐1 of the N‐RAS gene. Threonine (ACA) was substituted for alanine (GCA) at codon 11. This mutation would have been overlooked by conventional probe hybridization techniques. A search for other mutations in N‐RAS exon‐1 in T‐ALL revealed a codon 13 mutation substituting aspartic acid (GAT) for glycine (GGT) in one of 18 patients. No mutations at codon 12 were detected.