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Long‐term study of chimaerism in bone marrow transplantation recipients for severe aplastic anaemia
Author(s) -
Keable Hélène,
Bourhis JeanHenri,
Brison Olivier,
Lehn Pierre,
Schenmetzler Claudine,
Devergie Agnès,
Gluckman Eliane
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb06313.x
Subject(s) - bone marrow , minisatellite , transplantation , ex vivo , peripheral blood mononuclear cell , medicine , immunology , cyclophosphamide , bone marrow transplantation , biology , in vivo , chemotherapy , microsatellite , genetics , in vitro , gene , allele
S ummary . We used minisatellite probes to analyse by DNA fingerprints the long‐term engraftment (median 4.3 years, range 1–2) of 21 bone marrow transplantation recipients for severe aplastic anaemia. Patients received their graft from histocompatible siblings. They were conditioned with cyclophosphamide (150 mg/kg) and a 6GY thoracoabdominal irradiation and did not have ex‐vivo T cell depletion of marrow donor. DNA was extracted from peripheral mononuclear cells and analysed by Southern blotting with 32 Plabelled single‐stranded RNA probes. Seven out of 21 donor‐recipient pairs were sex‐mismatched and additionally studied with a probe detecting a male specific repeated sequence on the Y chromosome. Red cell surface phenotype was also used as marker of engraftment in most cases. Long‐term engraft‐ment appeared complete for all patients studied with respect to the three methods.