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Human monoclonal anti‐D antibodies. I. THEIR PRODUCTION, SEROLOGY, QUANTITATION AND POTENTIAL USE AS BLOOD GROUPING REAGENTS
Author(s) -
Kumpel B. M.,
Poole G. D.,
Bradley B. A.
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb06285.x
Subject(s) - monoclonal antibody , autoanalyzer , microbiology and biotechnology , antibody , antigen , serology , monoclonal , red blood cell , chemistry , typing , virology , immunology , biology , chromatography
Summary Eight monoclonal IgG antibodies to the Rh antigen D, produced by Epstein‐Barr virus transformed B‐lymphoblastoid cell lines from two individuals, have been assessed for their suitability as blood grouping reagents. All showed similar specificity and agglutinated all red cells with partial D antigens tested except category D VI cells. They gave strong reactions with D u red cells in indirect antiglobulin tests, and they all gave good reactions with R 1 R 1 , R 2 R 2 and R 0 r cells in manual tests using antiglobulin, enzyme or albumin methods. Initial studies showed that some of the monoclonal antibodies worked well at high dilutions on a Technicon Autogrouper 16C when used for routine D‐typing of blood donors. Antibody production by the cell lines was stable for many months in continuous culture. These monoclonal antibodies may be useful diagnostic reagents.