Premium
Heterogenous response of B cell chronic lymphocytic leukaemia (B‐CLL) cells to anti‐human IgM antibody (anti‐μ) and B cell stimulatory factor 1 (BSF‐1)
Author(s) -
Kawano Michio,
Iwato Koji,
Asaoku Hideki,
Tanabe Osamu,
Tanaka Hideo,
Ishikawa Hideaki,
Imamura Nobutaka,
Kuramoto Atsushi
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb06273.x
Subject(s) - surface immunoglobulin , chronic lymphocytic leukemia , b cell , antibody , immunology , microbiology and biotechnology , cell , cancer research , biology , chemistry , leukemia , genetics
Summary B cell chronic lymphocytic leukaemia (B‐CLL) cells from seven patients were examined for 3 H‐TdR uptake with anti‐human IgM antibody (anti‐μ) and B cell stimulatory factor 1 (BSF‐1)/IL‐4. B‐CLL cells from one patient could proliferate with anti‐μ plus BSF‐1, but not with BSF‐1 alone. B‐CLL cells from two patients responded to BSF‐1 alone; one could proliferate more by adding anti‐μ, but the proliferation of the other cells was inhibited by adding anti‐μ. On the other hand, B‐CLL cells from the other four patients responded neither to anti‐μ nor to anti‐μ plus BSF‐1. Surface densities of membrane IgM of B‐CLL cells were also analysed by fluorescence activated cell sorter (FACS), but there was no correlation between proliferative response (DNA synthesis) of B‐CLL cells to anti‐μ and densities of surface membrane IgM. These results show that there is a functional heterogeneity of B‐CLL cells with regard to proliferative response to anti‐μ and BSF‐1.