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Diagnostic differentiation of chronic B‐cell malignancies using monoclonal antibody L161 (CD1c)
Author(s) -
Jones Richard A.,
Master Peter S.,
Child J. A.,
Roberts Bryon E.,
Scott Colin Stephen
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb06272.x
Subject(s) - cd23 , b cell , chronic lymphocytic leukemia , antibody , monoclonal antibody , biology , immunoglobulin light chain , cd38 , pathology , lymphoproliferative disorders , tonsil , immunology , lymphoma , medicine , leukemia , immunoglobulin e , genetics , stem cell , cd34
Summary The expression of membrane CD1c, as defined by monoclonal antibody L161, was examined on malignant lymphoid cells from 191 cases of chronic lymphoproliferative disease and on eight ‘normal’ enriched tonsil B‐cell extracts. Of 79 cases of chronic lymphocytic leukaemia (CLL) studied, 77 showed low (<20% positive cells) CD1c expression whereas 63/71 (89%) cases of B‐PLL, HCL and B‐NHL showed increased CD1c+ (but not CD1a or CD1b) components. In contrast, malignancies corresponding to terminal stages of B‐cell differentiation (immunocytoma and myeloma) generally showed low CD1c expression as did lymphoid cells from 10 cases of post‐thymic malignancy. Although there was some correlation between the expression of membrane CD1c and immunoglobulin (SIg) light chain densities (P <0.001), it is relevant in diagnostic terms that seven cases of B‐NHL, with low SIg staining intensities more typically associated with CLL were CD1c+. CD1c expression was not, however, correlated with the presence of CD23 or FMC7 determinants but did show a similar pattern of expression to that previously reported for beta‐2 microglobulin. Determination of cellular CD1c by APAAP immunocyto‐chemistry confirmed the presence of higher antigen densities in malignant B‐cells at intermediate/late stages of differentiation and this interpretation was further supported by the finding that the majority of phenotypically mature tonsil B‐cells were also CD1c+. The determination of CD1c expression by malignant B‐cells may therefore be of particular value in the diagnostic differentiation of chronic lymphoproliferative disorders.