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The inhibitory effect of serum from hairy‐cell leukaemia patients on normal progenitor cells may disappear following prolonged treatment with α‐interferon
Author(s) -
Lauria Francesco,
Bagnara Gian Paolo,
Catani Lucia,
Gaggioli Licia,
Guarini Anna,
Raspadori Donatella,
Foa Robert,
Bellone Graziella,
Buzzi Marina,
Gugliotta Luigi,
Zauli Giorgio,
Giovannini Marco,
Luigi zinzani Pier,
Tura Sante
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb04312.x
Subject(s) - progenitor cell , inhibitory postsynaptic potential , hairy cell , population , bone marrow , in vitro , immunology , alpha interferon , alpha (finance) , medicine , interferon , endocrinology , biology , andrology , stem cell , hairy cell leukemia , leukemia , biochemistry , surgery , construct validity , genetics , environmental health , patient satisfaction
Summary The possibility that serum from hairy‐cell leukaemia (HCL) patients at diagnosis may show an inhibitory effect on the in vitro colony growth of normal haemopoietic progenitor cells has been suggested. Several studies have documented the efficacy of α‐Interferon (α‐IFN) in inducing a complete restoration of peripheral blood values and, in some cases, a complete clinical remission. In this study we have evaluated the regulatory effect of serum, collected before and after 3 and 12 months of a‐IFN tretment, from 10 patients with untreated HCL, on the in vitro growth of normal bone marrow CFU‐GM, BFU‐E and CFU‐MK. The effect of conditioned media, prepared from enriched hairy cells (HC) cultured in synthetic medium, on the growth of normal haemopoietic progenitors was also investigated. The results obtained confirm that sera from untreated HCL patients display a variable degree of inhibitory activity in the progenitor cell compartments analysed. Disappearance of the inhibitory activity, particularly evident for the erythroid compartment, was found only in patients who displayed a disappearance of circulating HC and a good haematological response after prolonged (12 months) treatment with a‐IFN. The possibility that the serum of patients with HCL may contain a haemopoietic inhibitory factor, released by the neoplastic HC population, is suggested.