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Polyclonal rearrangements of the T‐cell receptor β‐chain in fatal angioimmunoblastic lymphadenopathy
Author(s) -
Knecht Hans,
Odermatt Bernhard F.,
Hayoz Daniel,
Kühn Lukas,
Bachmann Fedor
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb00286.x
Subject(s) - t cell receptor , polyclonal antibodies , gene rearrangement , biology , germline , monoclonal , clone (java method) , southern blot , microbiology and biotechnology , angioimmunoblastic t cell lymphoma , lymphoma , pathology , t cell , antibody , monoclonal antibody , immunology , gene , medicine , genetics , immune system
Summary Genomic rearrangement of germline T‐cell antigen receptor (TcR) and immunoglobulin (Ig) genes was studied by Southern blot analysis in seven patients with angioimmunoblastic lymphadenopathy (AILD). In three cases clinically suspected of transformation into malignant lymphoma, hybridization with the TcRβ probe showed markedly dimished intensity in the 11.5 kb germline band after Eco RI digestion and normal germline configuration after Hind III and Bam HI digestion, indicating polyclonal T cell rearrangements. A clonal rearrangement of the TcRβ gene was detected in only one case at initial biopsy. No monoclonal rearrangement of Ig genes was observed. These data show that in some cases of AILD disease progression is indicated by polyclonal TcR rearrangements and not by outgrowth of a malignant clone, supporting the concept of AILD as an immunoregulatory disorder.