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No preferential use of the VH(V) family in human multiple myeloma
Author(s) -
Clofent G.,
Brockly F.,
Commes T.,
Lefranc M. P.,
Bataille R.,
Klein B.
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb00285.x
Subject(s) - lymphoblast , gene rearrangement , multiple myeloma , antibody , immunoglobulin heavy chain , chronic lymphocytic leukemia , microbiology and biotechnology , myeloma protein , biology , gene , cell culture , genetics , immunology , leukemia
Summary A recently described immunoglobulin VH family (the VH(V) family) close to the DH and JH genes is preferentially rearranged in immature B‐cell tumours. The question of the emergence of multiple myeloma (MM) from a tumorous pre‐B cell is not yet resolved. To draw a comparison with chronic lymphocytic leukaemia (CLL), we studied the VH(V) rearrangements in 28 MM patients. A rearranged Hind III‐Bam HI fragment of 9.5 kb was detected in only one patient instead of the rearranged fragment of 8.5 kb described in CLL. Rearrangements of a member of the VH(V) family in a 9.5 kb fragment were also observed in two out of 20 lymphoblastoid cell lines obtained from peripheral blood of MM patients. We report here that the VH(V) family is not preferentially involved in this pathology and that the size of the only rearrangement obtained is larger than the 8.5 kb fragment observed in CLL. These results do not favour the hypothesis of a pre‐B cell involvement in MM.