Premium
Identification of H‐ and L‐ferritin subunit binding sites on human T and B lymphoid cells
Author(s) -
Anderson G. J.,
Faulk W. Page,
Arosio P.,
Moss D.,
Powell L. W.,
Halliday J. W.
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb00262.x
Subject(s) - ferritin , protein subunit , microbiology and biotechnology , monoclonal antibody , recombinant dna , antibody , biology , chemistry , biochemistry , immunology , gene
Summary Monoclonal antibodies to the H and L subunits of ferritin were used in indirect immunofluorescent studies to investigate the subunit specificity of ferritin binding to transformed T and B lymphoid cell lines. Pre‐incubation of cells with recombinant H ferritin followed by antibodies to the H subunit produced a labelling pattern in which all cells showed strong fluorescent stippling. Corresponding experiments with liver ferritin and an antibody to the L subunit revealed that most cells were lightly stippled, but 3–5% of the cells were intensely labelled. These two labelling patterns were distinct as assessed by cross‐blocking experiments with recombinant H and liver ferritins. These results imply that different binding sites exist for each subunit.