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Co‐incident N and K ras gene mutations in a case of AML, restricted to differing cell lineages
Author(s) -
Hiorns Lynne R.,
Cotter Finbarr E.,
Young Bryan D.
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb00247.x
Subject(s) - microbiology and biotechnology , percoll , polymerase chain reaction , biology , mutation , gene , oligonucleotide , dna , genetics , in vitro
Summary Peripheral blood from a patient with acute myeloid leukaemia (AML) of M5 FAB classification, was shown to have mutations to both the N and K ras genes. Leucophoresed blood was separated on a discontinuous Percoll density gradient to provide fractions enriched for different cell lineages. DNA extracted from these fractions was amplified using the polymerase chain reaction (PCR) technique, and hybridized with oligonucleotide probes specific for the single base mutations previously demonstrated. The N‐ras mutation was shown to be restricted to the blast and monocytic cell fractions, concordant with the FAB subtype of M5. The K‐ras mutation, however, was present in all fractions, suggesting it had occurred in a multi‐potential stem cell representing an earlier stage in the generation of the leukaemia, or possibly an incidental background phenomenon.