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Human liquid bone marrow culture in serum‐free medium
Author(s) -
Drouet Xavier,
Douay Luc,
Giarratana MarieCatherine,
Baillou Claude,
Gorin NorbertClaude,
Salmon Charles,
Najman Albert
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb00243.x
Subject(s) - haematopoiesis , transferrin , stem cell , bone marrow , in vitro , bovine serum albumin , stromal cell , cell culture , fetal bovine serum , chemically defined medium , growth factor , immunology , biology , andrology , medicine , endocrinology , microbiology and biotechnology , biochemistry , genetics , receptor
Summary Prolonged in vitro maintenance of human bone marrow progenitor cells was achieved using a serum‐free (SF) liquid culture system. Culture medium was based on Iscove's medium supplemented with bovine serum albumin, human transferrin, bovine insulin, soybean lecithin, cholesterol, hydrocortisone and alpha‐thioglycerol. Under these standardized culture conditions, CFU‐GM were maintained for up to 4 weeks, as is the case when using conventional serum‐dependent medium. Erythropoiesis exhibited a slower decline than that found using serum containing medium. Development of normal haematopoiesis was effective in spite of poor stromal cell development—a confluent adherent layer as classically described in serum conditions was never achieved. Our newly defined system provides a reliable technique for studying human haematopoietic stem cell proliferation and differentiation in vitro ; it allows for rational utilization of currently available purified recombinant growth factors. It may be a promising tool in the clinical use of cultured haematopoietic stem cells.