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Rheological properties of erythrocytes in recombinant human erythropoietin‐administered normal rat
Author(s) -
Maeda Nobuji,
Kon Kazunori,
Tateishi Norihiko,
Suzuki Yoji,
Sekiya Misuzu,
Taniguchi Takuya,
Seike Masahiko,
Nakajima Takashi,
Shiga Takeshi,
Tanaka Kouichi,
Shinkura Hirofumi
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb00228.x
Subject(s) - reticulocytosis , erythropoietin , polycythaemia , endocrinology , medicine , chemistry , recombinant dna , blood viscosity , erythrocyte deformability , intracellular , red blood cell , hematocrit , echinocyte , anemia , biochemistry , gene
Summary. Recombinant human erythropoietin [rhEPO (180 iu/μg); 1 or 10 μg polypeptide equivalent/kg] was intravenously administered daily to 6‐week‐old normal rats for 1 week. Rheologically, (1) blood viscosity at shear rate of 40–380 s −1 increased in a manner entirely dependent upon the haematocrit, (2) no change in erythrocyte deformability was detected by high shear rheoscopy, and (3) the velocity of rouleau formation in autologous plasma (at 7.5 s −1 ) decreased. Haematologically, rhEPO administration induced considerable polycythaemia with reticulocytosis in a dosedependent manner, accompanying increased cell volume and decreased intracellular haemoglobin concentration, thus the density distribution of erythrocytes shifted towards low specific gravity. Plasma viscosity and plasma protein composition were unaffected by rhEPO‐administration. In erythrocyte metabolism, no drastic alteration in the level of 2,3–DPG or ATP was detected.