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Acetaldehyde‐modified serum proteins inhibit mitogen‐stimulated human lymphocyte transformation
Author(s) -
Wickramasinghe S. N.,
Barden G.
Publication year - 1989
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1989.tb00213.x
Subject(s) - acetaldehyde , pokeweed mitogen , phytohaemagglutinin , lymphocyte , albumin , chemistry , serum albumin , ethanol , biochemistry , in vitro , microbiology and biotechnology , biology , immunology , concanavalin a
Summary. Sera obtained from healthy volunteers immediately before and 8 h after the rapid consumption of 1.2 g ethanol/kg body weight were dialysed against RPMI 1640 and added ab initio to microcultures of normal human lymphocytes containing 1–3 μg phytohaemagglutinin (PHA)/ml or 2–8 μg pokeweed mitogen (PWM)/ml. When compared with the pre‐alcohol sera, the post‐alcohol sera inhibited lymphocyte transformation after 48 h incubation with either mitogen. In other experiments, acetaldehyde‐albumin complexes were generated by reacting solutions of human serum albumin with 45–720 μ m acetaldehyde, and the same quantity of either unmodified albumin or acetaldehyde‐modified albumin was included in freshly‐prepared lymphocyte microcultures containing 3 μg PHA/ml or 8 μg PWM/ml. When compared with unmodified albumin, acetaldehyde‐modified albumin inhibited lymphocyte transformation after 48 h of culture with either mitogen. The inhibition of lymphocyte transformation caused by post‐alcohol sera and acetaldehyde‐modified albumin was partially corrected after treatment of the proteins with 1.55 m m sodium borohydride at a pH of 9.5. The data indicate that post‐alcohol sera contain a non‐dialysable activity which inhibits mitogen‐stimulated lymphocyte transformation in vitro and that at least part of this activity may reside in acetaldehyde‐modified serum albumin.