Premium
Enrichment of haemopoietic progenitor cells from the marrow of patients with myelodysplasia
Author(s) -
Baines Paul,
Masters Gillian S.,
Lush Christopher,
Jacobs Allan
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.tb06183.x
Subject(s) - progenitor cell , clonogenic assay , myelodysplastic syndromes , myeloid , erythropoiesis , biology , bone marrow , cancer research , immunology , progenitor , in vitro , erythroblast , haematopoiesis , stem cell , microbiology and biotechnology , medicine , anemia , genetics
Summary Myeloid and erythroid progenitors from myelodysplastic marrows have been separated from accessory cell populations likely to influence their in‐vitro growth. Myeloid colony‐forming cells were enriched 23‐fold and erythroid progenitors 5‐7‐fold but, in both cases, retained their abnormal growth characteristics. After enrichment and removal of lymphocytes and monocytes, erythroid burst formation became markedly more dependent on the addition of 5637 bladder carcinoma conditioned medium as an exogenous source of haemopoietic growth factors. This suggests that lymphocytes and monocytes usually support erythropoiesis in cultures of myelodysplastic marrow and demonstrates that erythroid burst‐forming progenitor cells from myelodysplastic patients can respond to these populations in vitro. Haemopoietic failure in myelodysplasia appears to result from a defect within the preleukaemic clonogenic cell, rather than from an aberration in exogenous factors. The procedure outlined here can be used as a first step towards the isolation of these abnormal progenitors.