Premium
Effect of red cell age on vesiculation in vitro
Author(s) -
Greenwalt T. J.,
Dumaswala U. J.
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.tb04237.x
Subject(s) - vesicle , in vitro , red blood cell , in vivo , red cell , cholesterol , chemistry , blood cell , biology , biochemistry , immunology , medicine , membrane , genetics
Summary Microvesiculation of RBC occurs in vivo and during in vitro storage. Vesiculation of young and old populations of RBC was measured under blood bank conditions. The RBC in whole units of blood were divided by density into two approximately equal fractions in a closed system. After storage for 35 d the haemoglobin‐containing vesicles were quantitatively recovered. Quantitation of protein, cholesterol and phospholipids showed significantly larger amounts in the vesicles shed by the younger RBC. Otherwise vesicles shed by the two populations of RBC did not differ.