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Differential phthalocyanine photosensitization of acute myeloblastic leukaemia progenitor cells: a potential purging technique for autologous bone marrow transplantation
Author(s) -
Singer C. R. J.,
Linch D. C.,
Bown S. G.,
Huehns E. R.,
Goldstone A. H.
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.tb04228.x
Subject(s) - clonogenic assay , acute myeloblastic leukemia , bone marrow , medicine , progenitor cell , cancer research , transplantation , in vitro , leukemia , pathology , stem cell , immunology , chemistry , biology , biochemistry , genetics
Summary The potential value of sutphonated aluminium phthalocyanine (AISPc) as a purging agent for bone marrow autografts in acute myeloblastic leukaemia (AML) has been studied using in vitro clonogenic assays for normal (GM‐CFC) and leukaemic (AML‐CFC) progenitor cells. In nine out of 13 cases, the leukaemic blasts were found to be highly sensitive to AISPc. In six of the sensitive cases clonogenic assays revealed that only 2.1% of AML progenitor cells survived AISPc treatment under conditions which permitted a GMCFC recovery of 60.11%. AISPc photosensitization was also shown to selectively eliminate the leukaemic cell line K562 from an in vitro model of minimal residual disease. Thus photosensitization using AISPc may be an effective method of purging marrow autografts in some cases of AML. Evaluation of the sensitivity of AML clonogenic cells at diagnosis may identify those patients in whom AISPc photo‐purging may be of benefit at the time of an autologous bone marrow transplant.