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Monocyte‐derived macrophages as helper cells in monocyte‐mediated cytolysis
Author(s) -
Dallegri Franco,
Ballestrero Alberto,
Frumento Guido,
Goretti Riccardo,
Patrone Franco
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.tb04175.x
Subject(s) - cytolysis , monocyte , lysis , opsonin , in vitro , taurine , chemistry , zymosan , mononuclear phagocyte system , peripheral blood mononuclear cell , microbiology and biotechnology , biochemistry , biology , amino acid , immunology , cytotoxicity
Summary. Human peripheral blood monocytes (M), incubated with opsonized zymosan particles (OPZ), lysed human erythrocyte (RBC) targets, as detected by a 51 Cr release method. Conversely, cells derived in vitro from M (monocyte‐derived macrophages, MDM) were ineffective. When added to the M‐RBC system, MDM enhanced the lysis. The lysis by M and M plus MDM was prevented by catalase, azide and amino acids (alanine, taurine), consistent with the requirement for hypochlorous acid (HOCl). Moreover, MDM per se incapable of generating HOCl augmented the HOCl recovery from the M‐RBC system. The results provide evidence for a previously unrecognized form of interaction between two distinct populations of mononuclear phagocytes.