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Production of mouse monoclonal antibodies for the analysis of idiotypes in serum of patients with chronic lymphatic leukaemia
Author(s) -
De Rie Menno A.,
Van Heemstra Diederik J. M.,
Huijgens Peter C.,
Zeijlemaker Wim P.,
Out Theo A.,
Melief Cornelis J. M.,
Von Dem Borne Albert E. G. Kr.
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.tb04172.x
Subject(s) - idiotype , immunoglobulin d , antibody , immunology , immunoglobulin idiotypes , monoclonal antibody , spleen , b cell , monoclonal , immunoglobulin m , medicine , biology , microbiology and biotechnology , immunoglobulin g
Summary. In this report a simple procedure for the production of murine monoclonal antibodies (MoAb) against the idiotype of malignant B cells is described. Mice were immunized with lymphoid cells from patients with B‐cell chronic lymphocytic leukaemia (B‐CLL). After fusion of the spleen cells, hybridoma supernatants were screened for anti‐idiotypic MoAb in ELISA with immunoglobulins obtained from tumour‐cell lysates, xenohybridomas and patients' sera. The anti‐idiotypic MoAb were used to study tumour cells and serum immunoglobulins (Ig) from four different patients with B‐CLL. It was found that the serum IgM and IgD in one patient shared the same idiotype. Evidence is presented that IgG‐secreting cell populations are not restricted to λ‐Ig‐light chain‐expressing B‐CLL cells. With the help of anti‐idiotype MoAb accurate measurements of total and idiotype‐positive serum immunoglobulin levels during chemotherapy were possible.