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Functional studies of cell‐mediated immunity in haemophilia and other bleeding disorders
Author(s) -
Mahir Walla S.,
Millard Rosemary E.,
Booth J. C.,
Flute P. T.
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.tb02375.x
Subject(s) - phytohaemagglutinin , immunology , haemophilia , lymphocyte , medicine , cytomegalovirus , cellular immunity , von willebrand disease , antigen , haemophilia a , peripheral blood lymphocyte , herpesviridae , viral disease , platelet , virus , von willebrand factor , pediatrics
Defects of immunoregulation which occur in haemophilia, reflected by numerical changes in T lymphocyte subsets, have been further investigated in functional studies. Polyclonal T‐cell activation by the mitogen phytohaemagglutinin (PHA) and specific stimulation by cytomegalovirus (CMV) or herpes simplex type 2 (HSV‐2) in previously sensitized subjects were studied in peripheral blood lymphocyte and in T4‐cell‐enriched cultures. Compared with 12 controls, the responses of 11 patients (nine with haemophilia A and two with von Willebrand's disease) to PHA were significantly reduced both in unfractionated and in T4‐cell‐enriched peripheral blood lymphocyte cultures. Reduced responses to PHA were found in HIV (HTLV III)‐seronegative as well as ‐seropositive patients. There were no significant differences between the response of patients’unfractionated and T4‐enriched peripheral blood lymphocytes to CMV/HSV‐2 recall antigen and the control subjects, although there was evident variation in the magnitude of patients’unfractionated and T4‐enriched lymphocyte responses.