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The measurement of the total volume of red cells in man: a non‐radioactive approach using biotin
Author(s) -
Cavill I.,
Trevett D.,
Fisher J.,
Hoy T.
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.00491.x
Subject(s) - biotin , flow cytometry , streptavidin , red cell , labelling , volume (thermodynamics) , chemistry , biotinylation , cell , nuclear medicine , microbiology and biotechnology , chromatography , radiochemistry , medicine , biochemistry , biology , physics , quantum mechanics
Present methods for measuring red cell volume are based on the dilution of radioactively labelled cells. This precludes the investigation in neonates and pregnant women. We present a simple method for labelling red cells with biotin. These cells may be injected intravenously and subsequently detected using streptavidin‐FITC and flow‐cytometry. A comparison of the red cell volume estimated using both 51 Cr and biotin labelled cells in 19 patients showed no consistent clinically significant difference between the two. This novel label appears to allow red volume to be reliably estimated without using radioactivity.