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Establishment and characterization of a permanent human IgA 2 /kappa myeloma cell line
Author(s) -
Lohmeyer J.,
Hadam M.,
Santoso S.,
Förster W.,
Schulz A.,
Pralle H.
Publication year - 1988
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1988.00321.x-i1
Subject(s) - microbiology and biotechnology , immunoglobulin d , immunoglobulin light chain , antigen , biology , plasma cell , calla , antibody , clone (java method) , cd19 , cd38 , epitope , transferrin receptor , b cell , monoclonal antibody , flow cytometry , transferrin , immunology , cd34 , biochemistry , stem cell , dna
A human myeloma cell line designated LOPRA‐1 has been established from ascites fluid containing malignant plasma cells of a patient with IgA 2 /kappa multiple myeloma. The cultured cells which are Epstein‐Barr virus (EBV) negative have retained the morphological, cytochemical, ultrastructural and immunophenotypical features of well‐differentiated plasma cells. They express the plasma cell antigen PCA‐1, the antigens CD28 (Kolt‐2) and CD38 (OKT10), the transferrin‐receptor (OKT9), and some epitopes of the CD24 antigen (HB8, VIB E3), but are negative for surface immunoglobulins, HLA class II antigens (HLA‐DP, ‐DQ, ‐DR) and other B‐cell markers such as CD10 (CALLA), CD19 (B4), CD20 (B1), CD21 (B2), CD22 (HD39), CD23 (MHM6), CD37 (BL14) and CD39 (G28‐8) as analysed by both flow cytometry and immunocytochemistry (PAP/APAAP). With respect to immunoglobulin synthesis, two stable clones were selected by single cell cloning: clone LOPRA‐1/5 synthesizes large amounts of alpha 2 heavy and kappa light chains, but secretes only small amounts of these molecules, whereas clone LOPRA‐1/4 is clearly devoid of intracellular immunoglobulin heavy and light chains and thus appears to be a chain loss variant. Cytogenetic analysis revealed a pseudotriploid phenotype with several structurally abnormal marker chromosomes: 3n+ ‐, 70, XX, ‐X, ‐1, ‐4, ‐6, ‐8, ‐8, ‐13, ‐16, +7, +18, +21, +i(1q), +i(1q), +6q‐, +3mar.