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Decreased sialic acid content of erythrocytes in patients with aplastic anaemia
Author(s) -
Yoshida Minore,
Takaku Fumimaro,
Nojiri Hlsao,
Miura Yasusada,
Saito Masaki
Publication year - 1987
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1987.tb06867.x
Subject(s) - sialic acid , sialidase , thiobarbituric acid , hemolysis , platelet , aplastic anemia , chemistry , neuraminidase , immunology , medicine , biochemistry , virus , enzyme , lipid peroxidation , bone marrow
Summary. The sialic acid content of erythrocytes from patients with aplastic anaemia was determined and compared with those in patients with several haematological disorders and healthy individuals. The sialic acid was released enzymatically with Vibrio cholerae sialidase and quantitated by the thiobarbituric acid method (Aminoff, 1961). The sialic acid content of normal erythrocytes was 538 ±31 nmol/ml of packed erythrocytes. That of erythrocytes from patients with aplastic anaemia was 480±35 nmol/ml of packed erythrocytes, which was significantly lower than normal (P<0.01). In contrast, erythrocytes from patients with myeloproliferative disorders showed significantly (P<0.05) higher sialic acid contents (564±45 nmol/ml of packed erythrocytes). These results suggest that some membrane changes occur in erythrocytes in patients with these disorders.