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Heterogeneity of IgG1 monoclonal anti‐Rh(D): an investigation using ADCC and macrophage binding assays
Author(s) -
Armstrong Sylvia S.,
Wiener Edith,
Garner S. F.,
Urbaniak S. J.,
Contreras Marcela
Publication year - 1987
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1987.tb01308.x
Subject(s) - antibody dependent cell mediated cytotoxicity , monoclonal antibody , monoclonal , immunology , microbiology and biotechnology , chemistry , medicine , biology , antibody
Summary Three monoclonal IgG1 anti‐Rh(D), UCH D4, ARC 7D5 and UKTS FC3, produced by Epstein‐Barr virus transformed cells from Rh(D)‐sensitized individuals, were compared with polyclonal single donor anti‐D sera and therapeutic immunoglobulin preparations in antibody dependent cellular cytotoxicity (ADCC) and macrophage binding tests. When assayed at equal anti‐D concentrations monoclonal antibodies varied considerably in their ADCC and macrophage binding activities: only UKTS FC3 showed significant activity in both assays, but these were substantially lower than those of the polyclonal anti‐D sera and immunoglobulins. When examined in different combinations the monoclonal antibodies showed little synergism in mediating red cell destruction by the effector cells. Factors which might contribute to the diverse ADCC and macrophage binding activities of the monoclonal anti‐Ds of the same IgG subclass are discussed.