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Phenotypic and genotypic analysis of chronic myelogenous leukaemia with T lymphoblastic and megakaryoblastic mixed crisis
Author(s) -
Yasukawa Masaki,
Iwamasa Kikue,
Kawamura Shinichi,
Murakami Satoshi,
Takada Kiyonori,
Hato Takaaki,
Shiosaka Takahiko,
Tamai Tomonori,
Fukuoka Tamotsu,
Fujita Shigeru,
Kobayashi Yuzuru
Publication year - 1987
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1987.00331.x
Subject(s) - megakaryocyte , bone marrow , biology , immunology , phenotype , chronic myelogenous leukemia , lymph node , lineage (genetic) , genotype , pathology , microbiology and biotechnology , cancer research , leukemia , genetics , medicine , gene , haematopoiesis , stem cell
Summary A case of blast crisis in chronic myelogeneous leukaemia (CML) in which two distinct cell lineages were involved is presented. The phenotype of blasts in lymph nodes was T11 (CD2) + , Ia + , TdT + , suggesting T cell lineage. On the other hand, blasts in bone marrow and peripheral blood expressed platelet glycoprotein IIb/IIIa complex on their surface, suggesting megakaryocyte lineage. Cytogenetic analysis of lymph node and bone marrow cells revealed the abnormalities. inv(7) (p15q34) and t(1;3) (q23;q21), respectively, as well as the presence of the Ph 1 chromosome in both cell types. Rearrangement of the T cell receptor β‐chain gene was detected in lymph node blasts, although blast cells in peripheral blood showed a germ line configuration. The involvement of T cell and megakaryocyte lineages in the blast crisis phase of CML was confirmed in our phenotypic and genotypic analysis, and the pathogenic association between blast crisis lineages and the additional chromosome abnormalities present is discussed.