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Cloning of human erythroid progenitors (BFU‐E) in the absence of fetal bovine serum
Author(s) -
Migliaccio Giovanni,
Migliaccio Anna Rita
Publication year - 1987
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1987.00129.x
Subject(s) - clone (java method) , cloning (programming) , yolk sac , progenitor cell , biology , embryonic stem cell , cell culture , microbiology and biotechnology , fetus , stem cell , in vitro , fetal bovine serum , immunology , andrology , embryo , genetics , medicine , gene , pregnancy , computer science , programming language
Summary We describe culture conditions which enabled us to clone early human erythroid progenitors (BFU‐E) in the absence of fetal bovine serum (FBS). Our medium, which is chemically fully defined, supports proliferation and differentiation of erythroid progenitors comparable to that in FBS‐supplemented cultures. Furthermore, it allows cloning of BFU‐E from all human haemopoietic tissues (adult marrow, embryonic liver and yolk sac) and adult or perinatal blood. This system should facilitate the investigation of human erythroid differentiation in vitro (e.g. cell‐cell interaction. mechanism of action of haemopoietins and inhibitors, regulation of Hb synthesis) as well as the mechanisms underlying myeloproliferative disorders. As an example, we have found that recombinant Ep shows, in this culture system, the same dose response as Ep from conventional sources.