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Von Willebrand factor contaminating porcine factor VIII concentrate (Hyate: C) causes platelet aggregation
Author(s) -
Altieri Dario C.,
Capitanio Anna M.,
Mannucci Pier Mannuccio
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb07554.x
Subject(s) - von willebrand factor , platelet , platelet aggregation , von willebrand disease , medicine , immunology
S ummary Severe thrombocytopenia has been reported to occur in some patients treated with a polyelectrolyte‐fractionated porcine factor VIII concentrate (Hyate:C) (Kernoff et al , 1984; Gatti & Mannucci, 1984). We report here that Hyate: C induces aggregation of platelet‐rich plasma in vitro accompanied by ATP release and thromboxane B 2 formation, and is inhibited by EDTA and prostaglandin E 1 . Hyate:C induced platelet aggregation is affected by monoclonal antibodies anti‐glycoprotein Ib or anti‐glycoprotein IIb/IIIa and suppressed by polyclonal anti‐porcine von Willebrand factor antiserum. We conclude that the aggregating ability of Hyate :C is due to porcine von Willebrand factor present in the concentrate. The occasional thrombocytopenia described after Hyate: C infusions probably results from the interaction between platelets and the porcine von Willebrand factor contaminating the concentrate.