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The myelosuppressive effect of recombinant interferon γ in short‐term and long‐term marrow cultures
Author(s) -
Coutinho L. H.,
Testa N. G.,
Dexter T. M.
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb07529.x
Subject(s) - haematopoiesis , progenitor cell , bone marrow , stromal cell , granulocyte , immunology , biology , myeloid , stem cell , microbiology and biotechnology , cancer research
S ummary The effect of a highly purified human gamma IFN (r‐γ‐IFN) on the growth of haemopoietic progenitors was examined in soft agar assays and in long‐term bone marrow cultures. r‐γ‐IFN reduced colony formation by progenitor cells of the granulocyte/macrophage lineage (GM‐CFC). the eryth‐roid lineage (BFU‐E) and multipotent cells (GEMM‐CFC), and suppressed haemopoiesis in long‐term culture in a dose‐related fashion. At high doses (1000 units/ml) r‐γ‐IFN appeared to be toxic to the stromal cells of the bone marrow.