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Alloantibody‐induced platelet serotonin release is blocked by antibody to the platelet PL antigen
Author(s) -
Duncan J. R.,
Rosse W. F.
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb04126.x
Subject(s) - platelet , apyrase , serotonin , chemistry , fibrinogen , antigen , antibody , secretion , endocrinology , microbiology and biotechnology , medicine , immunology , pharmacology , biochemistry , biology , receptor
S ummary . Anti‐platelet antibodies are known to activate the platelet release mechanism, usually measured by [ 14 C]serotonin secretion. We have made immunoglobulin preparations of antibodies to HLA antigens, blood group A and platelet‐specific P1 A1 antigen on the platelet surface and have defined the optimal conditions for [ 14 C]serotonin secretion by washed platelets. For optimal [ 14 C]serotonin release, platelets were washed in apyrase and suspended in Tyrode‐albumin with Ca ++ and Mg ++ and fibrinogen. Anti‐P1 A1 at high concentration did not cause [ 14 C]serotonin release and inhibited serotonin release by other alloantibodies to antigens on the platelet surface. This was shown to be a result of the inhibition of fibrinogen binding to the platelet by bound anti‐P1 A1 .