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Fibronectin‐mediated inhibition of human platelet activation by crosslinked immunoglobulin G
Author(s) -
Sypcher M. O.,
Lerch P. G.,
Nydegger U. E.
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb04123.x
Subject(s) - fibronectin , platelet , chemistry , stimulation , receptor , antibody , platelet activation , immunoglobulin g , platelet aggregation , serotonin , microbiology and biotechnology , pharmacology , immunology , biochemistry , endocrinology , medicine , biology , cell
S ummary . The effect of purified human plasma fibronectin on stimulation of human platelets with crosslinked immunoglobulin G (IgG) was tested by means of aggregometry, 14 C‐serotonin release and fibronectin‐to‐platelet binding experiments. Incremental additions of fibronectin to gel‐filtered platelets (8x10 8 /ml) followed by 25 μg/ml bisdiazoniumbenzidine (BDB)‐crosslinked IgG produced a dose‐related inhibition of platelet aggregation and suppression of 14 C‐serotonin release from the platelets. Evidence was obtained that upon stimulation of the platelets with BDB‐IgG, fibronectin becomes bound to its receptor and that further platelet activation is inhibited when a sufficient number of receptors is occupied.