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A monoclonal antibody reactive with a subset of human plasma cells
Author(s) -
Gonchoroff Nick J.,
Katzmann Jerry A.,
Garton John P.,
RuizArgüelles Guillermo J.,
Eilers Carol R.,
Greipp Philip R.,
Kyle Robert A.
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb04084.x
Subject(s) - monoclonal antibody , flow cytometry , antibody , microbiology and biotechnology , plasmacytoma , bone marrow , plasma cell , spleen , antigen , biology , multiple myeloma , chemistry , immunology
Summary A mouse monoclonal antibody (Rl‐3) raised against a human plasmacytoma xenograft and reactive with human plasma cells is described. The antibody reacts with a subset of plasma cells and lymphocytes, but is nonreactive with granulocytes, normoblasts, thymus, spleen or T lymphocytes. Flow cytometer double labelling experiments showed that approximately 50% of Rl‐3 positive bone marrow cells expressed surface immunoglobulin, but no Rl‐3 positive cells also expressed B1 or Leu 4. In a myeloma patient the R1‐3 reactive cells were found to have a higher rate of DNA proliferation and were aneuploid as determined by flow cytometry. The R1‐3 antigen is expressed on late B lymphocytes and early plasma cells.