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An association between the factor VII coagulant activity and thrombin activity induced by surface/cold exposure of normal human plasma
Author(s) -
Miller G. J.,
Seghatchian M. J.,
Walter S. J.,
Howarth D. J.,
Thompson S. G.,
Esnouf M. P.,
Meade T. W.
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb02941.x
Subject(s) - factor vii , chemistry , factor x , thrombin , coagulation , medicine , endocrinology , factor v , platelet , thrombosis
S ummary . To test whether factor VII activation correlated with the generation of thrombin activity when plasma was exposed to a glass surface and reduced temperature, an association was sought between the changes in factor VII clotting activity (VII c ) and fibrinopeptide A concentration (an index of thrombin activity) in platelet‐poor citrated plasma from 42 healthy adults. The Spearman rank correlation ( r s ) between responses was 0.82 ( P < 0.001). The VII c assay response to surface/cold exposure was unaffected when thrombin was suppressed by hirudin. An assay for factor VII activity based upon its activation of tritiated factor X revealed an association between the increase in fibrinopeptide A concentration and reduction in functional factor VII concentration during activation of a subset of 22 plasma samples ( r s = ‐0.62; ( P < 0.003). This loss of functional factor VII was probably due to conversion of active factor VII to its non‐functional end‐product by factor X a . The results suggest that VII c is an index of flux within the coagulation system and support the hypothesis that a high VII c is an indicator of a hypercoagulable state.