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Cellular levels of mRNA from c‐ myc , c‐ myb and c‐ fes onc ‐genes in normal myeloid anderythroid precursors of human bone marrow: an in situ hybridization study
Author(s) -
Emilia Giovanni,
Donelli Amedea,
Ferrari Sergio,
Torelli Umberto,
Selleri Licia,
Zucchini Patrizia,
Moretti Luigi,
Venturelli Donatella,
Ceccherelli Giovanni,
Torelli Giuseppe
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb02931.x
Subject(s) - myeloid , bone marrow , myb , microbiology and biotechnology , in situ hybridization , biology , messenger rna , gene , gene expression , rna , cancer research , immunology , genetics
S ummary . The expression of three onc ‐genes, c‐ myc , c‐ myb and c‐ fes , has been evaluated at the cellular level in myeloid and erythroid precursors of normal human bone marrow, by ‘ in situ ’ hybridization with tritium‐labelled probes. A relatively large amount of m‐RNA from the three onc ‐genes was detected in myeloblasts and promyelocytes, but whereas the expression of c‐ myb and c‐ myb decreased in more advanced stage of maturation of the myeloid lineage, c‐ fes mRNA remained at a relatively high level until the granulocyte stage, c‐ myc and c‐ myb were expressed at a fairly high level in basophilic erythroblasts, which also showed low levels of c‐ fes mRNA. No expression of these onc ‐genes was detectable in more mature erythroblasts, Megakaryocytes showed high levels of m‐RNA from all three onc ‐genes. Our results suggest that c‐ myc and c‐ myb expression is related in some way to the cellular proliferation of myeloid and erythroid precursors, whereas c‐ fes expression is more restricted to myeloid differentiation.