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Fluorescence probe measurement of the pH of the transferrin microenvironment during iron uptake by rat bone marrow erythroid cells
Author(s) -
Veldman A.,
Heul C.,
Kroos M. J.,
Eijk H. G.
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb02912.x
Subject(s) - transferrin , endocytosis , intracellular , vesicle , chemistry , fluorescence , biochemistry , transferrin receptor , bone marrow , incubation , biophysics , microbiology and biotechnology , cell , biology , immunology , membrane , physics , quantum mechanics
S ummary . Fluorescence probe measurements of the transferrin micro‐environment during iron uptake by rat erythroid cells revealed that part of the transferrin is taken up in an acidic environment. The pH of this intracellular transferrin environment is 5·7. When rat erythroid cell precursors are incubated with diferric transferrin then in the incubation medium monoferric transferrins Tf N Fe and TfFe c appear. In view of the known instability of Tf N Fe at acidic pH, Tf N Fe cannot arise after endocytosis of Tf 2 Fe in acid vesicles at pH below 6·0. The results support the existence of a mechanism other than endocytosis in the iron uptake process in rat erythroid cells.