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In vitro growth of sheep erythroid progenitors
Author(s) -
Kelly Susan J.,
Wood W. G.
Publication year - 1986
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1986.tb02238.x
Subject(s) - in vitro , fetus , bone marrow , andrology , progenitor cell , immunology , biology , medicine , endocrinology , physiology , pregnancy , stem cell , microbiology and biotechnology , genetics
S ummary . We have attempted to define culture conditions suitable to support the in vitro growth of early erythroid progenitors (BFU‐E), derived from the liver or bone marrow and blood of fetal and adult sheep. Testing a wide variety of conditions showed that only some batches of fetal lamb sera made a notable difference to growth. BFU‐E from normal fetal blood were demonstrated sporadically; they appeared regularly in low numbers in blood taken during erythroid (anaemic) stress. Adult blood only contained sporadic BFU‐E during severe anaemic stress. This difficulty in demonstrating circulating BFU‐E in sheep may be due to a genuine paucity of such cells or to an undefined requirement for in vitro growth.