Premium
The red cell antigens A, B, D, U, Ge, Jk3 and Yt a are not detected on human granulocytes
Author(s) -
Gaidulis Laima,
Branch Donald R.,
Lazar Gary S.,
Petz Lawrence D.,
Blume Karl G.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb07470.x
Subject(s) - antigen , microbiology and biotechnology , immunology , biology
S ummary We report the inability to detect the following red blood cell antigens on human granulocytes: A, B, D, U, Gerbich (Ge), Jk a Jk b (Jk3) and Cartwright (Yt a ). To study each antigen, granulocytes were purified on density gradients, fixed in glutaraldehyde, and the uptake of specific antisera measured using two direct immunological techniques: 125 I‐staphylococcal protein A ( 125 I‐SPA) binding and avidin‐biotin‐complex (ABC) immunoperoxidase staining. Glutaraldehyde fixation was shown not to affect the antigenicity when the antisera were tested using red blood cells. Using three anti‐A, three anti‐B and three anti‐A,B antisera, our 125 I‐SPA results of 47 tests with granulocytes from group A individuals and 39 tests with granulocytes from group B individuals indicate that A or B antigens are not expressed on human granulocytes. Tests using ABC were also negative with 37 and 36 granulocytes from group A or B individuals, respectively. In addition, no positive results using 125 I‐SPA were obtained with granulocytes from individuals having antigen positive red cells when tested with two anti‐D (number of tests performed ( n = 22), three anti‐Ge ( n = 22), three anti‐U ( n = 20), two anti‐Jk3 ( n = 17), and three anti‐Yt a ( n = 25); control anti‐NA1 or ‐NB1 antisera were invariably positive. Also, using these antisera, no positive results were obtained by ABC except with one anti‐Yt a antiserum which was positive with one of seven granulocytes tested. This anti‐Yt a was also positive with three of 10 granulocytes by 125 I‐SPA. This activity was shown to be due to a granulocyte‐specific antibody; adsorption of the antiserum with human granulocytes removed all activity against granulocytes but did not reduce the activity against red cells. Thus, our results are in agreement with recent reports which demonstrated the absence of the A, B and D antigens on human granulocytes. However, we have been unable to confirm previous reports which indicated the