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Regulation of human peripheral blood BFU‐E growth in vitro by leukaemic B‐lymphocytes
Author(s) -
Najman Albert,
Baillou Claude,
Drouet Xavier,
Leblanc Ginette,
Douay Luc,
Claude Gorin Norbert,
Duhamel Gearard
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb07468.x
Subject(s) - peripheral blood , peripheral blood mononuclear cell , in vitro , immunology , haematopoiesis , microbiology and biotechnology , biology , chemistry , stem cell , biochemistry
S ummary We report a stimulating effect of leukaemic B‐lymphocytes from anaemic and non‐anaemic patients with CLL on the proliferation of normal peripheral blood BFU‐E. Coculture of leukaemic B‐cells at various concentrations (2.5 x 10 3 ‐10 6 ) with 2.5 x 10 5 mononuclear cells from normal peripheral blood increased the number of BFU‐E derived erythroid colonies. The same effect was observed when the number of target cells was varied in the presence of a fixed number of B‐lymphocytes, with a clear linear relationship. B‐cell conditioned medium gave a similar increase when added to the culture instead of B‐cells. At high concentration of B‐cells from anaemic patients, the size of the colonies was increased and a large number of macroscopic colonies was seen. The place of the B‐cells in the regulation of erythroid progenitors in relation to monocytes and T‐lymphocytes has still to be established.