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Studies on the locomotory behaviour and adhesive properties of mononuclear phagocytes from blood
Author(s) -
Lackie J. M.,
Urquhart C. M.,
Brown A. F.,
Forrester J. V.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb07455.x
Subject(s) - peripheral blood mononuclear cell , fibronectin , adhesion , monocyte , mononuclear phagocyte system , in vitro , adhesive , albumin , chemistry , penetration (warfare) , in vivo , immunology , cell , microbiology and biotechnology , biology , biochemistry , organic chemistry , layer (electronics) , operations research , engineering
S ummary Mononuclear phagocytes isolated from peripheral blood move slowly on albumin‐, collagen‐ and fibronectin‐coated glass, as judged by analysis of time‐lapse film, although their movement is stimulated somewhat by serum. When a differential‐adhesion method is used to purify monocytes from a crude mononuclear cell fraction it appears to select a particularly slow‐moving sub‐group. Despite their slow rate of movement (especially in comparison with neutrophils) monocytes move over and penetrate monolayers of endothelial cells, apparently without difficulty; they are, however, restricted to the upper surface of a fibroblast monolayer. Penetration of reconstituted collagen gels by freshly isolated monocytes was not observed but cultured monocytes, which spontaneously detached from the culture substratum over a 48 h period, did invade collagen gels. The adhesive properties of these cultured cells, measured in a flow‐chamber assay, did not differ from freshly isolated monocytes purified on serum‐coated dishes and detached with EDTA, and their invasive capacity does not seem to be a consequence of altered adhesive properties. The behavioural differences between monocytes and neutrophils are considerable, although both cell types have to leave the circulation and penetrate tissues in vivo .