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Effects of cetiedil on the oxidative metabolism of activated polymorphonuclear leucocytes
Author(s) -
Kohzaki Hitoshi,
Kakinuma Katsuko,
Asakura Toshio,
Yamakawa Tamio
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb07450.x
Subject(s) - respiratory burst , chemistry , nadph oxidase , phagocytosis , enzyme , opsonin , oxygen , oxidative phosphorylation , oxidase test , biochemistry , lysozyme , liberation , biophysics , biology , in vitro , immunology , organic chemistry
S ummary Cetiedel, α‐cyclohexyl‐3‐thiopheneacetic acid 2‐ (hexahydro‐lH‐azepin‐1‐yl)‐ethyl ester, was found to specifically suppress oxygen uptake by polymorphonuclear leucocytes (PMN) that were exposed to myristate or heat‐killed E. coli . The chemical had no effect on the basal respiration rate of PMN in the resting state. Inhibition of oxygen uptake by cetiedil was proportionate to the degree of inhibition of the generation of O‐ 2 and H 2 O 2 . It was also found that cetiedil suppressed the rate of the phagocytosis by PMN of opsonized oil droplets. Cetiedil had no effect on subcellular NADPH oxidase, an enzyme responsible for the respiratory burst that is activated by the perturbation of PMN plasma membrane with phagocytable particles or stimulators such as myristate. These results suggest that cetiedil affects the trigger mechanism of the plasma membrane to inhibit the activation of NADPH oxidase.