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Megakaryocytic cells in mixed haemopoietic colonies (CFU‐GEMM) from the peripheral blood of normal individuals
Author(s) -
Ganser Arnold,
Elstner Elena,
Hoelzer Dieter
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb07357.x
Subject(s) - peripheral blood mononuclear cell , progenitor cell , monoclonal antibody , bone marrow , immunology , biology , microbiology and biotechnology , in vitro , stem cell , precursor cell , monoclonal , peripheral blood , blood cell , antibody , chemistry , biochemistry
S ummary The cellular composition of mixed haemopoietic colonies CFU‐GEMM from the peripheral blood of normal individuals was investigated using both an improved in vitro culture assay and a monoclonal antibody reactive with the earliest megakaryocytic precursor cells. The frequency of CFU‐GEMM was found to be 22± 7/ml blood (mean± SE; n = 8) and 4.9± 1.8/10 6 mononuclear cells. Double‐labelling of single colonies with monoclonal antibodies against megakaryocytic as well as granulocytic cells revealed that only 18% of mixed colonies from the peripheral blood contained megakaryocytic cells as compared to 87% of mixed colonies from the bone marrow, while no difference existed with regard to granulocytic cells. It is concluded that circulating multilineage progenitor cells either require different culture conditions to express their megakaryocytic component or, alternatively, that the circulating pluripotent stem cell pool mainly consists of bipotent rather than multipotent progenitor cells.

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