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The flow of blood cell suspensions through 3 μm and 5μm Nuclepore membranes: a comparison of kinetic analysis with scanning electron microscopic examinations
Author(s) -
Jones J. G.,
Holland B. M.,
Humphrys J.,
Wardrop C. A.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb07341.x
Subject(s) - membrane , scanning electron microscope , red blood cell , biophysics , filtration (mathematics) , chemistry , blood cell , materials science , biology , composite material , biochemistry , immunology , statistics , mathematics
S ummary This study was designed to investigate the effect of red and white cells on the flow of dilute suspensions of blood cells through 3 μm and 5 μm Nuclepore membrane filters. The rate of flow of blood cell suspensions through 3 μm or 5 μm membranes declines continually due to occlusion of pores by slow cells. The cells which occlude 3 μm pores exceed the number of white cells about seven‐fold. Electron microscopic examination of a used membrane confirms that these slow cells are not white cells but are red cells which are visibly damaged. With 5 μm membranes, the number of slow cells is entirely consistent with them being white cells. Again, electron microscopic examination confirms that 5 μm pores are occluded by white cells. With both types of membrane, the same kinetic analysis is valid and yields information about the behaviour of red cells during the filtration procedure.