Premium
Investigation of a coagulation accelerating factor (CAF) in glomerulonephritis
Author(s) -
Salem Hatem H.,
Howard Margaret A.,
Koutts Jerry,
Firkin Barry G.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb07335.x
Subject(s) - coagulation , von willebrand factor , coagulopathy , factor ix , thrombin , immunology , medicine , glomerulonephritis , chemistry , endocrinology , von willebrand disease , microbiology and biotechnology , platelet , biology , kidney
S ummary A coagulation accelerating factor was purified from the plasma of two patients with glomerulonephritis (GN) who suffered from thrombotic complications. The factor co‐purified with factor VIII/von Willebrand factor complex (FVIII/vWf) and under dissociating conditions remained associated with the factor VIII coagulant activity (FVIII). Control purified FVIII/vWf showed no coagulation accelerating activity under the experimental conditions used. The levels of coagulation accelerating factor, FVIII and von Willebrand factor (vWf) were reduced by incubation with rabbit anti‐human FVIII/vWf or human anti‐FVIII serum indicating a close association of these three activities. Multimeric analysis of the plasma FVIII/vWf complex from the two patients demonstrated a reduction in the high molecular weight multimers and the presence of an additional band not present on analysis of normal FVIII/vWf. It is sugggested that the coagulation accelerating factor represents an active form of FVIII which has different in vitro properties to thrombin activated FVIII.