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Detection of the blastic crisis cell clone in chronic myeloid leukaemia
Author(s) -
Löwenberg Bob,
Hagemeijer Anne,
Abels Johannes
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb02960.x
Subject(s) - clone (java method) , chronic myeloid leukaemia , blast crisis , immunology , myeloid , karyotype , cell culture , biology , cell , medicine , cancer research , genetics , gene , chromosome
S ummary. Three patients with Ph 1 positive chronic myeloid leukaemia (CML) were followed until the occurrence of blastic transformation by means of cytogenetic analysis of whole marrow or blood cells, of blast cell enriched fractions from marrow or blood and of colonies grown from these cell fractions in two culture systems. Karyotypic evolution was used to indicate the development of new clones from pre‐existing cell lines either as transient phenomena during the chronic phase or as the ultimate indicators of a blastic crisis (BC). The clones which were to lead to a BC were detected some months before the clinical diagnosis of the BC. At a time before overt BC had become clinically manifest, the BC clone outgrew the other ones in culture and thus also in vitro showed specific proliferative advantages over the cell lines characteristic of the chronic phase of CML.