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The effect of retinoids on CFU‐GM from normal subjects and patients with myelodysplastic syndrome
Author(s) -
BaileyWood R.,
May S.,
Jacobs A.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb02958.x
Subject(s) - clone (java method) , retinoic acid , myelodysplastic syndromes , retinoid , medicine , endocrinology , tretinoin , incubation , biology , bone marrow , biochemistry , gene
S ummary. Five retinoid analogues differ in their effects on the growth of CFU‐GM from normal human marrow. At concentrations above 10 −6 M all have inhibitory activity. This is maximal with 13‐cis‐retinoic acid. At lower concentrations the effect of retinoids is usually to reduce clone size rather than clone numbers though 10 −8 ‐10 −7 M etretinate increases clone size. The incubation of marrow cultures in the presence of 10 −6 M all‐trans retinoic acid showed that in normal subjects colony counts are never reduced to less than 45% of the control value and total clone counts to less than 70% of the control value. In cultures from patients with myelodysplastic syndrome 20 out of 34 cases showed a greater inhibition of colony numbers than normal and 14 out of 35 cases showed a greater reduction in total clone numbers than normal. The results suggest that CFU‐GM from some patients with myelodysplastic syndrome may have a greater sensitivity than normal to the inhibitory effect of retinoic acid.